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KMID : 1144820220480040334
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대한의생명과학회지
2022 Volume.48 No. 4 p.334 ~ p.339
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Evaluation of Loop Mediated Isothermal Amplification Based Methods for the Detection of African Swine Fever Virus from Food Waste
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Lee Si-won
Kwon Jun-hwa
Kim Su-Hyang
Kim Jin-Ho
Jung Jae-won
Lee Kyung-Jin
Park Ji-Yeon
Choi Taek-Kyun
Kang Jun-Gu
Han Tae-Uk
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Abstract
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African swine fever virus (ASFV) is a highly contagious and lethal pathogen that poses a threat to the global pork industry. The World Organization for Animal Health (WOAH) has placed strict surveillance measures for ASFV. The possibility of long-term survival of ASFV in raw meat or undercooked pork has been reported. Accordingly, the problem of secondary infection in food waste from households or waste disposal facilities has emerged, raising the need for ASFV monitoring of food waste. However, most of the previously reported ASFV gene detection methods are focused on clinical monitoring of pigs. There are very few cases in which their application in waste has been verified. Since ASFV diagnosis requires rapid monitoring and immediate action, loop-mediated isothermal amplification (LAMP) may be suitable, but this requires conformity assessment for LAMP to be used as a diagnostic technique. In this study, six LAMP methods were evaluated, and two methods (kit and manual) were recommended for use in diagnosing ASFV in food waste.
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KEYWORD
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African swine fever virus, ASFV, Food waste, LAMP
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